Homologous recombination for universal donor cells and chimeric mammalian hosts

摘要

Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the beta 2-microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs.