| 摘要 |
PURPOSE:To prevent aggregation and sedimentation reactions in blending a dyeing reagent of a biosample by a method wherein a pH buffer solution and a stabilizer are added to an azo dye and a xanthene dye as pigment for dyeing cells and structures to make the dye reagent of the biosample. CONSTITUTION:One pt.vol. of about 0.2 to 10X1/10<2>mol/l Evans blue or typan blue is mixed with about 0.5-2 pts.vol. of about 0.2 to 10X1/10<2>mol/l Erythrosine. Sodium azide, parahydroxyphenyl acetate, or dehydroacetate is mixed into the solution to obtain its 0.01-1% solution. The solvent used is a phosphoric acid solution, a succinic acid solution, a trisic acid solution of about 1/30-1/5mol% in concentration, and pH is kept at about 5.7-7.9. As fixing agent, any of glutaric aldehyde, formaldehyde and the like is added to be 0.02-5% in concentration. This makes the dyeing of a tangible component specifically and the obtaining of a dye image with high visual recognizability possible. |
