A process for preparing embryonic stem cell-like cells ("ES-like cells") is described. The process includes providing a source of primordial germ ("PG") cells, and a source of feeder cells of a suitable type; contacting the PG cells with the feeder cells; culturing the PG cells in a culture medium; and identifying and isolating ES-like cells from the culture medium; wherein the culture step is performed in the absence of exogenous growth factors and/or exogenous cytokines.
申请公布号
WO9510599(A1)
申请公布日期
1995.04.20
申请号
WO1994AU00624
申请日期
1994.10.13
申请人
THE UNIVERSITY OF MELBOURNE;CHERNY, ROBERT, ALAN;BRANDON, MALCOLM, ROY;WILLIAMS, ROBERT, LINDSAY