IMPROVED METHOD FOR RADIOLABELING MONOVALENT ANTIBODY FRAGMENTS

摘要

A method for radiolabeling a monovalent Fab or Fab' antibody fragment which comprises the steps of cleaving a divalent F(ab)2 or F(ab')2 antibody fragment using sulfite or meta-bisulfite ions to produce a monovalent fragment having a free sulfhydryl group, and contacting the monovalent fragment with a cation of a metallic radionuclide which binds to sulfhydryl groups, without removing excess sulfite or metabisulfite.