发明名称 |
Ribosome inactivating proteins (RIB), their method of production and medical preparation containing RIP. |
摘要 |
<p>Ribosome inactivating protein (RIB) having a mol mass of 31 kDa+/- 0.4 DA (by SDS-PAGE), an IEP of 8 to 9 and the N-terminal aminoacid sequence Asp-Ile-Ala-Leu-Asp-Leu-Ser-Thr- Ala-Thr-(Lys)-Ala-Ser-Tyr- Ser-Ala-Phe-Ile-Thr-His-(Trp)-Arg-Asn, is claimed. Also claimed is a RIB isolation method by (a) isolation of plant parts, in particular of seeds of Cucurbita varieties, and their pulverisation by grinding; (b) extn. of (a) with lipophilic, organic solvents; (c) extn. of the dried residue with a salt contg. buffer; (d) enrichment of proteins from (c); (e) enrichment of the basic protein fractions by anionic exchange chromatography; and (f) purificn. by gel filtration of the fractions active in the ribosome inhibition test.</p> |
申请公布号 |
EP0645399(A2) |
申请公布日期 |
1995.03.29 |
申请号 |
EP19940104611 |
申请日期 |
1994.03.23 |
申请人 |
LOMAPHARM RUDOLF LOHMANN GMBH KG PHARMAZEUTISCHE FABRIK |
发明人 |
WAGNER, HILDEBERT, PROF.DR.;ERFLE, VOLKER, PROF.DR.;GERHAEUSER, CLARISSA, DR.;OTT, HOLGER |
分类号 |
C07K14/415;(IPC1-7):C07K14/415;A61K38/16 |
主分类号 |
C07K14/415 |
代理机构 |
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代理人 |
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主权项 |
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