DETERMINATION OF NUCLEIC ACID BY PCR, MEASUREMENT OF NUMBER OF MICROBIAL CELLS, GENES, OR GENE-COPIES BY PCR, AND MEASURING-KIT EMPLOYED FOR THE SAME

摘要

A PCR amplification product is detected, in quantitative determination of nucleic acid and measurement of the number of bacterial cells or specific genes, by addition of a dye compound which does not fluoresce in the free state but fluoresces in the bonded state to a double-stranded nucleic acid.