| 摘要 |
<p>A method of quantifying the frequency of cells containing a selected nucleic acid sequence in a suspension of cells heterogenous with respect to the presence of the sequence, comprising (a) determining the total number of cells in the suspension, (b) dispensing the suspension into a plurality of vessels in order to provide a series of multiply replicated aliquots at decreasing concentrations of cells, (c) determining the presence or absence of the selected sequence in each vessel, and (d) relating the number of vessels containing the selected sequence to the total number of cells assayed at each dilution, such that the frequency of cells containing the selected nucleic acid sequence in the suspension may be determined.</p> |
