Pretreating reagent, pretreatment method, assay with pretreated samples, assay kit and method of diagnosing infectious diseases

摘要

A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, beta -glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulus reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.