Method for assaying nucleic acids and proteins using anthracene derivative phosphate

摘要

Method for assaying nucleic acids and proteins employing no radiosotope labeling, which is suitable for data storage and allows to carry out the detection efficiently and high sensitivity. The sample selectable from the group of nucleic acids, proteins, and other chemical compounds is adhered on a nylon membrane filter to be bound to a phosphatase. Then the phosphatase is reacted with an anthracene derivative phosphate followed by irradiating the reaction product with ultraviolet light to detect a fluorescence emitted therefrom.