RECOMBINANT DNASE B DERIVED FROM STREPTOCOCCUS PYOGENES

摘要

The gene for Streptococcus pyogenes DNase B has been cloned and vectors incorporating the cloned DNA have been used to transform Escherichia coli, allowing production of the DNase in E. coli. The enzyme can be produced with a leader peptide at its amino terminus. Method for the purification of naturally occurring S. pyogenes DNase B enzyme is also provided. The DNase B enzyme produced, either by purification of naturally occurring enzyme or by recombinant DNA techniques, can be used to generate antibodies and can also be used in immunochemical assays to detect the presence of anti-DNase B antibodies in serum as a marker of infection by S. pyogenes.