发明名称 |
Cloning vector |
摘要 |
A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.
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申请公布号 |
US5376549(A) |
申请公布日期 |
1994.12.27 |
申请号 |
US19930014944 |
申请日期 |
1993.02.05 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
GUILFOYLE, RICHARD A.;SMITH, LLOYD M. |
分类号 |
C12N15/70;(IPC1-7):C12N15/70 |
主分类号 |
C12N15/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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