ENHANCED EXPRESSION OF PROTEIN BY USING CULTURE MEDIUM CONTAINING MEMBRANE LIPIDS

摘要

The invention relates to microorganism culture medium for the expression of protein which comprises membrane interactive lipids or membrane lipids belonging to class II or III polar lipids or precursors thereof, such as monoolein or ethoxylated vitamin E or inositol, in an amount of more than 50 mg/L. The invention also relates to a method for expressing protein in recombinant microorganisms which is characterised by the addition of the membrane interactive lipid or membrane lipids or precursors. The microorganism is preferably yeast and the protein is preferably IGF-I. The invention also relates to the use of the membrane interactive lipids or membrane lipids belonging to class II or III polar lipids or precursors thereof in an amount of more than 50 mg/L as enhancer for the expression of protein in a microorganism culture medium.