FUSION PROTEINS CONTAINING ADENO-ASSOCIATED VIRUS REP PROTEIN AND BACTERIAL PROTEIN

摘要

A fusion protein including an adeno-associated virus rep protein or a fragment or derivative thereof, and a protein or peptide which is not an adeno-associated virus protein or peptide. Such fusion protein may be produced by genetic engineering techniques wherein there is provided an expression vehicle including a first DNA sequence encoding an adeno-associated virus rep protein or a fragment or derivative thereof, and a second DNA sequence encoding a protein or peptide which is not an adeno-associated virus protein or peptide, whereby expression of said first DNA sequence and said second DNA sequence results in expression of a fusion protein including the adeno-associated virus rep protein or fragment or derivative thereof and the protein or peptide, such as the E. coli maltose-binding protein, which is not an adeno-associated virus protein or peptide. Such fusion proteins may be produced and purified in large quantities while the virus rep protein portion of the fusion protein retains its biological activity.