Apparatus for pretreating cells for flow cytometry.

摘要

A plurality of open-top sample tubes (16), each containing a sample liquid containing cells to be treated, are removably mounted on a disklike sample carrier (14) which is rotatable about a vertical axis for centrifuging the samples. Disposed above the sample carrier are a set of fixed reagent supply nozzles (46) for dropping a desired reagent or reagents into the sample tubes, and at least one supply-discharge nozzle (48) movable into and out of the successive sample tubes. A hydropneumatic circuit (63; 163; 363) is coupled to the supply-discharge nozzle (48) for intimately intermingling the samples and the reagents and for rinsing the supply-discharge nozzle and other required parts. In an alternate embodiment a single supply-discharge nozzle (48) is employed for reagent supply into the sample tubes, for intermingling the samples and the reagents, and for rinsing.