| 摘要 |
<p>Devices and methods for carrying out an assay for the determination of nucleic acids are disclosed. The devices comprise (a) a sample receiving chamber, (b) a plurality of additional chambers, at least one of the additional chambers containing reagents for carrying out an assay for the determination of a nucleic acid, (c) means for detecting a signal generated by the reagents, (d) means for permitting fluid communication between the additional chambers, (e) means for introducing a sample into the device, and (f) means for separating the analyte from the remainder of the sample and introducing the analyte into one of the additional chambers, which means comprises suspendible particles. The methods involve introducing a sample suspected of containing a nucleic acid analyte into a device in accordance with the invention. The analyte, if present, and particles in a medium are transported through the chambers of the device. Reagents for conducting an amplification are combined with the transported medium, which is subjected to conditions for amplifying the analyte. The medium is then examined for the presence of amplified analyte.</p> |
