METHOD FOR THE EXPRESSION OF GENES IN YEAST, AND DNA FRAGMENTS AS WELL AS PLASMIDS COMPRISING SAID DNA FRAGMENTS TO BE USED WHEN CARRYING OUT THE METHOD

摘要

A method of expressing genes in yeast by introducing into a yeast cell a recombinant DNA segment containing both the gene to be expressed and a 5' flanking region comprising a promoter sequence, and culturing of the transformed yeast cells in a growth medium, using as the recombinant DNA segment a segment, of which the 5' flanking region comprises a first DNA fragment containing a promoter sequence in combination with a second DNA fragment containing a leader sequence regulated on the posttranscriptional level by heme, heme analogs, or heme precursors. Furthermore DNA-fragments and plasmids to be used when carrying out the method. When carrying out the method according to the invention an increased expression of genes is obtained by a reduced genetic load of the host cell and a more optimal utilization of the protein synthesis apparatus and the energy metabolism.