Method of producing rice cybrid cells

摘要

A method of producing rice cybrid cells includes the steps of irradiating with X-rays to first rice protoplasts containing a first useful gene in the cytoplasm thereof to selectively destroy the nuclei thereof only; treating second rice protoplasts containing a second useful gene in the nucleus thereof with about 10 - 30 mM of iodoacetamide, about 0.1 - 0.5 mM Rhodamine 6G or about 10 - 30 mM iodoacetate to selectively deactivate the cytoplasm; and fusing the treated first and second protoplasts to form cybrid cells which have only the nucleus containing the first useful gene and have only the cytoplasm containing the second useful gene.