发明名称 |
CELL FREE SYSTEM FOR PROTEIN SYNTHESIS AND USE OF CHAPERONE PROTEINS THEREIN |
摘要 |
The present invention provides a novel high efficiency method for the cell free engineering and synthesis of protein. A novel method of the present invention comprises the steps of: preparing a cell free extract; separating out a ribosome fraction from said extract; incubating said ribosome fraction in the presence of a transcription/translation medium; and measuring the amount of protein synthesized. The method of the present invention may be used as a coupled transcription/translation system, a translation only system or a cell-free continuous flow system. Also provided are methods for synthesis of proteins and their correct folding using chaperone proteins.
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申请公布号 |
CA2159899(A1) |
申请公布日期 |
1994.10.27 |
申请号 |
CA19942159899 |
申请日期 |
1994.04.08 |
申请人 |
RESEARCH DEVELOPMENT FOUNDATION |
发明人 |
KUDLICKI, WIESLAW;KRAMER, GISELA;HARDESTY, BOYD |
分类号 |
C12N15/09;C12P21/02;(IPC1-7):C12P21/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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