| 发明名称 |
Reduced temperature dna denaturation using glycerol |
| 摘要 |
Method for determining the nucleotide base sequence of a double-stranded closed circular DNA. The method includes heating a solution which includes the DNA in the presence of at least 20% glycerol and/or ethylene glycol to at least 80 DEG C. to provide denatured DNA, and then sequencing the denatured DNA. |
| 申请公布号 |
AU6622194(A) |
申请公布日期 |
1994.10.24 |
| 申请号 |
AU19940066221 |
申请日期 |
1994.03.29 |
| 申请人 |
UNITED STATES BIOCHEMICAL CORPORATION |
发明人 |
CARL W FULLER;DENISE A PISA-WILLIAMSON |
| 分类号 |
G01N33/50;C07C31/18;C07C31/20;C12Q1/68 |
主分类号 |
G01N33/50 |
| 代理机构 |
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代理人 |
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| 主权项 |
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| 地址 |
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