发明名称 Determination of substrate of coenzyme requiring enzyme
摘要 PCT No. PCT/GB91/01781 Sec. 371 Date Mar. 17, 1993 Sec. 102(e) Date Mar. 17, 1993 PCT Filed Oct. 14, 1991 PCT Pub. No. WO92/07266 PCT Pub. Date Apr. 30, 1992.The invention relates to a competitive binding assay technique for substances (e.g. small molecules having no antigenic determinant) which can serve as substrates for coenzyme-requiring enzymes. The technique involves contacting a liquid sample with an assay system including (i) a substrate analogue and (ii) a moiety capable of binding specifically to the substrate without catalyzing the conversion of substrate to product. The substrate analogue is a substance capable of binding to said moiety competitively with any of the substrate present in the sample. The presence or amount of the substrate is determined by monitoring the extent to which there are formed or remain complexes between the moiety and substrate or substrate analogue.
申请公布号 US5356780(A) 申请公布日期 1994.10.18
申请号 US19930030151 申请日期 1993.03.17
申请人 APPLIED RESEARCH SYSTEMS HOLDING N.V. 发明人 ROBINSON, GRENVILLE A.;HURRELL, JOHN G.
分类号 C12Q1/26;C12Q1/00;C12Q1/32;G01N5/02;G01N21/78;G01N27/327;G01N33/542;G01N33/543;G01N33/573;G01N33/58;(IPC1-7):C12Q1/00;G01N33/53 主分类号 C12Q1/26
代理机构 代理人
主权项
地址
您可能感兴趣的专利