| 摘要 |
A method for determining the amount of analyte in a sample utilizes a series of test regions with systematically varied, preferably monotonically increasing, affinity for a specific binding partner for an analyte or for the analyte itself. By determining the portion of test regions which are capable of binding specific binding partner in competition with the analyte in the sample, or which bind the analyte, the amount of analyte may be estimated. Thus, titration of affinity replaces titration of concentration, allowing assays to be performed without the need for serial dilution steps. Alternatively, advantage is taken of competition of various ligands for components of complexes to regulate the release of desired components from the complexes. In one embodiment, clearance of small moieties can be delayed by combining them with size-enhancing agents which can be released or dissociated on cue by action of a releasing agent. In other embodiments, a target itself is a successful competitor in releasing the active form of the moiety from the complex. |
