| 摘要 |
PURPOSE:To determine a specimen with no influence of nonspecific agglutination by bringing a particle carrier sensitized by an immunoactive substance into contact with the specimen reactive specifically on the active substance and detecting variation of Zeta potential before and after contact. CONSTITUTION:Latex particles are employed as particle carrier and the type thereof is not limited. Various available antigens or antibodies are employed as immunoactive substance. Sodium chloride or potassium chloride dissolved into phosphoric acid buffer is employed as an electrolytic aqueous solution. According to normal immunoassay, the particle carrier sensitized by the immunoactive substance is suspended in the electrolytic aqueous solution and after measuring Zeta potential of the suspension, a specimen reactive specifically on the active substance is added to cause reaction and Zeta potential after reaction is measured. The specimen is determined based on the relationship between the potential difference before and after reaction and the concentration of the specimen. Since the specimen can be measured at low concentration, nonspecific agglutination can be suppressed during stock or measurement. |
