Sialic acid glycosides, antigens, immunoadsorbents, and methods for their preparation

摘要

The stereochemistry of sialylation of an acceptor saccharide to obtain an alpha (2-3) or alpha (2-6) linkage is controlled to favor the alpha anomer by use of an aromatic ester of the sialyl reagent. The resulting intermediate alpha (2-3) and alpha (2-6) sialylated intermediate disaccharide blocks are useful in the synthesis of antigenic substances which can be used to raise antibodies useful in diagnosis and therapy, and can themselves be used as reagents in various applications. The preparation of the tetrasaccharide antigens corresponding to the 19-9 and sialyl-X antigens characteristic of malignant tissue illustrates the application of this method.