ASUPERUGIRUSUORIZANIOKERUTANPAKUSEISEIBUTSUNOSEIZOHO

摘要

Proteins (I) are expressed in Aspergillus oryzae by first transforming the host with a recombinant DNA cloning vector which can integrate into the genome (in one or more copies) and which comprises (1) sequences facilitating gene expression; (2) a selection marker, and (3) the sequence coding for (I). The host does not contain a gene for the selection marker. The transformants are then grown in a suitable culture medium to produce (I). Also claimed is a promoter for protein expression in Aspergillus; it comprises the TAKA-amylase promoter (or funtional parts), opt. preceded by upstream activating sequences. Two pref. base sequences for this promoter are reproduced in the specification (about 1200 bases).