| 摘要 |
PURPOSE:To obtain a culture medium capable of not reducing the ratio of regeneration even in the application of a sever treatment for conversion to a protoplast by making up a double layer fixed medium of a layer containing carrageenan and a polyamine and another layer containing agarose. CONSTITUTION:A double layer fixed culture medium is made up of a layer containing carrageenan and a polyamine and another layer containing agarose. The former is used as the upper layer and the later is used as the lower layer in general. As the polyamine, spermidine, spermine, putrescine, etc., are exemplified. |
