| 摘要 |
Methods for performing in vitro assays to detect and quantitate the effects of anti-cancer agents on cancer cells are provided. In the methods of the invention, mammalian cancer cells are cultured in the presence of a single anti-cancer drug agent, or a combination of anti-cancer drug agent, in culture medium free of heterologous serum. After culturing the cells with the anti-cancer drug agent, Atp from the cells is extracted and stabilized, and measured using luminescence techniques, as illustrated in the figure. Kits for performing in vitro assays to detect and quantitate the effects of anti-cancer agents on cancer cells are also provided. |
