| 摘要 |
PCT No. PCT/EP93/03364 Sec. 371 Date Jun. 1, 1995 Sec. 102(e) Date Jun. 1, 1995 PCT Filed Dec. 1, 1993 PCT Pub. No. WO94/13135 PCT Pub. Date Jun. 23, 1994A method for the preservation of in vitro expanded epithelial sheets by incubation of the sheets in a cryopreserving solution characterized by lack of volume increase at the liquid-solid phase transition. The cryopreserving solutions comprise: a) polyethyleneglycols having molecular weight between 3 and 5 KD; and b) at least one cross-linking agent selected from polyols, polyamines, mono- or oligosaccharides, and polyethyleneglycols having molecular weight lower than 1 KD. |
