SOLID CULTURE MEDIUM AND THE METHOD OF ITS PRODUCTION

摘要

This solid nutrient medium for cultivating microorganisms contains a copolymer of acrylonitrile and acrylamide and a liquid nutrient substrate in the following percent-by-weight proportions: copolymer of acrylonitrile and acrylamide: 5.0 - 30.0; nutrient substrate: 70.0 - 95.0. The method of obtaining a solid culture medium includes the purification of acrylamide and N,N'-methylene-bis-acrylamide by recrystalization; preparation of the reactive mix composed of acrylonitrile, acrylamide, N,N'-methylene-bis-acrylamide, an acceptable solvent and an initiating system; polymerization over a period sufficient for the production of a copolymer retaining the form; its rinsing and saturation with water until equilibrium state; permeation with the nutrient substrate, where the components have these percent-by-weight proportions: acrylonitrile 5.0 - 50.0; acrylamide 5.0 - 50.0; N,N'-methylene-bis-acrylamide 0.01 - 0.60; initiating system 0.15 - 0.3; acceptable solvent residuum.