发明名称 |
PRODUCTION OF RECOMBINANT EUCALYOTE ALKALI PHOSPHATASE HAVING BIOLOGICAL ACTIVITY |
摘要 |
In a process for the production of recombinant, biologically active, eukaryotic alkaline phosphatase a DNA sequence coding for a eukaryotic alkaline phosphatase is expressed in a prokaryotic host cell and the expression product is obtained in an active form by cell lysis, solubilization under denaturing conditions and subsequent renaturation. In this process the renaturation step is carried out in the presence of one or several stabilizing agents. |
申请公布号 |
JPH06153938(A) |
申请公布日期 |
1994.06.03 |
申请号 |
JP19930190503 |
申请日期 |
1993.07.30 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
UUBUE MIHAERISU;RAINAA RUDORUFU;MIHAERU YARUSHIYU;ERUHAATO KOPETSUTSUKII;HERUMUTSUTO BAATOSHIYAA;GUNTAA SHIYUMAHIYAA |
分类号 |
C07K1/107;C12N9/16;C12N9/96;C12N15/09;C12N15/55;C12N15/70;C12R1/19;(IPC1-7):C12N9/16 |
主分类号 |
C07K1/107 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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