| 摘要 |
PURPOSE:To efficiently obtain the subject oligosaccharide useful for inclusive agents, etc., by reacting inulin with a treated microbe capable of producing the subject enzyme, produced by culture in a culture fluid containing inulin and specific amount of organic nitrogen source. CONSTITUTION:A culture fluid containing inulin and 0.01-1.0wt.% of organic nitrogen source is inoculated with microbes capable of producing the subject enzyme [e.g. Bacillus circulans MCI-2554 bacteria (FERM P-11940)] followed by shaking culture at 27 deg.C for 30 hr, and the resultant culture fluid is centrifuged to eliminate the microbes and reparate a culture filtrate, which is used as a crude fluid and is added to a solution containing 25wt.% of inulin and 50mM phosphate buffer solution (pH 7.0) followed by reaction at 37 deg.C for 10 hr, thus efficiently, and economically obtaining the objective cyclic inulooligosaccharide greatly high in solubility to water, and useful as a new inclusive agent. |
