发明名称 |
Voltammetric sequence-selective sensor for target polynucleotide sequences |
摘要 |
The present invention relates to a voltammetric sequence-selective sensor for target polynucleotide sequences which essentially comprises: an immobilized polynucleotide probe having one end covalently bound onto an amperometric electrode. The immobilized probe includes a target binding region capable of hybridizing to the target polynucleotide sequences forming an immobilized heteroduplex having at least a hybridized region. The sequence-selective sensor of the present invention also comprises means for voltammetrically detecting immobilized heteroduplexes. There is also provided a method for detecting the presence of a target polynucleotide analyte in a physiological sample, which comprises the steps of incubating the prepared physiological sample with the voltammetric sequence-selective sensor of the present invention; voltammetrically detecting immobilized heteroduplexes; and comparing the resulting voltammogram with a control voltammogram.
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申请公布号 |
US5312527(A) |
申请公布日期 |
1994.05.17 |
申请号 |
US19920957602 |
申请日期 |
1992.10.06 |
申请人 |
CONCORDIA UNIVERSITY |
发明人 |
MIKKELSEN, SUSAN R.;MILLAN, KELLY M.;SPURMANIS, ALEKSANDRS J. |
分类号 |
C12Q1/68;G01N33/543;(IPC1-7):G01N27/26 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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