LTD4 receptor and photoaffinity probe

摘要

The LTD4 receptor has been defined by radioligand binding studies as a member of the family of G-protein coupled receptors. A photoactivable azido derivative of LTD4 ([125I]Azido-LTD4) has been synthesized for use as a photoaffinity probe. Photoactivation of ([125I]Azido-LTD4 under equilibrium binding conditions revealed the selective radiolabeling of a 45 kDa protein in guinea-pig lung membranes, as visualized by SDS-PAGE and autoradiography.