Mutant AOX2 promoter, vector carrying same, transformant, and production of heterologous protein.

摘要

The application discloses a mutant AOX2 promoter obtained by mutating a sequence of the natural AOX2 promoter in a manner comprising at least one of the three mutation modes of (1) a region extending upstream from nucleotide 1187 inclusive and comprising at least nucleotides 845-960 is deleted, (2) (a) nucleotide(s) is(are) replaced in (a) region(s) of nucleotides 1274-1314, and (3) new oligonucleotide(s) is(are) inserted in (a) region(s) of nucleotides 1274-1314; vectors carrying said mutant AOX2 promoter; transformants into which said vector has been introduced; and methods for producing a heterologous protein, which comprise cultivating said transformant. The promoter of the present invention has a remarkably enhanced activity as compared with the natural AOX2 promoter and is highly useful as a promoter to be carried in an expression vector allowing heterologous protein expression. In addition, the vector and the transformant of the invention can efficiently express and produce various useful heterologous proteins.