摘要 |
<p>The production of polypeptide by the steps of (i) cloning a nucleic acid sequence by means of the polymeric chain reaction (ii) transforming an organism with the cloned nucleic acid sequence (iii) culturing the organism, is characterised by purifying or assaying the polypeptide with antibody or other specific binding reagent having specific affinity to a sequence of amino acids coded by a 'primer' nucleic acid sequence utilised in the polymerase chain reaction.</p> |