| 摘要 |
Thermostable sequence-specific DNA endonucleases are encoded by archaeal type introns of stable RNA (ribosomal RNA or transfer RNA) or protein genes or are enzymatically active variants thereof in which one or more amino acid residues have been deleted, inserted or substituted by other amino acids. These endonucleases recognize relatively long sequences of about 20 base pairs and are very rare cutters, cleaving with a frequency of about 1:5 000 000. Thus, they are useful as endonuclease tools for gene analysis, such as genome mapping and detection of major rearrangements in large genomes, and for gene manipulation, such as cloning and chromosome targeting. |
