Nucleotide vector comprising a translation inhibition factor gene, and IRES region and a desired gene.

摘要

1. A method for the in vitro production of a gene product, comprises: (a) preparing one or more recombinant nucleotide vector(s) capable of eukaryotic cell transfection, collectively containing 1) a gene encoding an eukaryotic translation inhibition factor of an eukaryotic translation factor (ETF), 2) at least one internal ribosome entry site (IRES) region, and 3) at least one gene encoding a polypeptide which it is desired to produce; (b) transfecting a eukaryotic cell culture with the said vector; (c) keeping the transfected cell culture under conditions suitable to promote cell activity; and (d) recovering the desired gene product from the said culture. <IMAGE>