发明名称 |
CHEMILUMINESCENT METHOD FOR THE QUANTITATION OF HUMAN DNA |
摘要 |
RAN 4095/94 The present invention provides methods and reagents for the estimation of the quantity of human DNA contained in a sample. Immobilized sample DNA is hybridized to a biotinylated oligonucleotide probe that hybridizes to a human genomic or mitochondria DNA. sequence. The subsequent binding of streptavidin-horseradish peroxidase to the bound probe allows for chemiluminescent detection using a luminol-based reagent and X-ray film. In addition, the present invention provides methods and reagents to assess the quality of DNA contained in a sample. The sample is first size fractionated by agarose gel electrophoresis, and then immobilized, hybridized to a biotinylated oligonucleotide probe, and detected using the chemiluminescent method as used in the quantity estimation methods of the present invention.
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申请公布号 |
CA2104767(A1) |
申请公布日期 |
1994.03.01 |
申请号 |
CA19932104767 |
申请日期 |
1993.08.24 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
REYNOLDS, REBECCA L.;WALSH, P. SEAN |
分类号 |
C12N15/09;C12Q1/68;G01N33/53;(IPC1-7):C12Q1/68;C07H21/00 |
主分类号 |
C12N15/09 |
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