发明名称 |
Factor viii purification process |
摘要 |
There is provided in accordance with the practice of this invention a process for separating Factor VIII complex from an impure protein fraction containing Factor VIII complex. An aqueous solution of the impure protein fraction containing Factor VIII complex is applied to a heparin-coupled chromatographic medium, to bind the Factor VIII complex to the medium. The Factor VIII is then recovered from the heparin-coupled chromatographic medium by elution with an aqueous solution comprising CaCl2 and histidine. The recovered Factor VIII is further purified by precipitation with a solution comprising glycine and NaCl, and washing the resultant precipitate with a solution comprising histidine, glycine, and NaCl to provide a Factor VIII complex solution with a specific activity of about 70 to about 150 units/mg.
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申请公布号 |
US5288853(A) |
申请公布日期 |
1994.02.22 |
申请号 |
US19920876410 |
申请日期 |
1992.04.30 |
申请人 |
ALPHA THERAPEUTIC CORPORATION |
发明人 |
BHATTACHARVA, PRABIR;MOTOKUBOTA, TOSHIHARU |
分类号 |
C07K14/755;(IPC1-7):A61K35/14;C07K13/00 |
主分类号 |
C07K14/755 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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