In vitro culture method for a fertilized ovum of a hen

摘要

A fertilized ovum is recovered from an upper portion of a magnum of an oviduct of a dead hen 60 to 80 minutes after oviposition. The fertilized ovum is placed in a container containing a thin albumen. A yolk of the ovum is covered with a sheet of gauze, and the ovum is cultured up to the blastoderm stage (stage X) in the container which is covered with a sheet of plastic film that is held by a rubber band.