| 摘要 |
An improved method is provided for diagnosis of cryptosporidiosis, an important zoonosis involving farm animals, using novel oligonucleotide probes and polymerase chain reaction primers specific to (C.parvum) and (C.baileyi). Probes and primers which differentiate between the two are also provided. Ligase chain reaction (LCR) may also be applied. The specific oligonucleotide probes and primers of the invention can be applied to oocysts in samples such as surface water, faeces etc. when a preferred embodiment which breaks these open is applied. |
