PLANT TISSUE CULTURE METHOD OF REGENERATION AND PROCEDURES FOR ISOLATING PROTOPLASTS

摘要

The invention relates to a method of regeneration of plant materials by cocultivation with microspores or anthers or cultivation in a medium containing extracts of microspores or anthers or a medium in which there has been cultured microspores or anthers or a medium containing fractions of a medium in which there has been cultured microspores or anthers. Further the invention relates to a method of isolating egg protoplasts and synergid protoplasts from ovules by mechanical dissection and a method for producing transgenic plants, homozygous plants, hybrids or cybrids and the plants, hybrids and cybrids produced by the method.