| 摘要 |
<p>The invention relates to a biospecific multiparameter assay method based on the use of different categories of microspheres representing different analytes to be assayed, said categories comprising different amounts of an internal fluorescent dye with short decay time, each category of microspheres being coated with a different biospecific reactant. The method comprises pooling the different categories of microspheres together in a suspension, adding the sample containing analytes to be assayed, adding a mixture of labelled biospecific reactants, diluting the suspension to reduce the concentration of labelled reactants not bound to the microspheres, and identifying the category of each microsphere on the basis of the strength of the electrical signal resulting from the short decay time fluorescent dye. According to the invention, the label of the biospecific reactant is either a compound generating or catalysing a chemi- or bioluminescence, or a phosphorescent compound.</p> |
