摘要 |
An system for imaging an oxygen-containing animal includes an illuminating light source 10, optical filters 12, a microscope and an associated epifluorescence attachment 16, a long pass cutoff filter 8, a camera 20, a frame grabber 22, a counter timer board 24, a computer 26 and an analog monitor 28. The light from the flash lamp 10 is passed through the optical filters to remove an unwanted portion of the spectrum, and focused on a sample object 14 through the epifluorescence attachment and phosphorescence is observed through the long pass cutoff filter. The images are collected with the camera and the frame grabber is used to digitize and average frames while the timing of the flash and gating of the camera intensifier is controlled by the counter timer board. The frames are displayed on the analog monitor.
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