发明名称 |
Transgenic tomato plants with altered polygalacturonase isoforms. |
摘要 |
<p>A method of creating a transgenic tomato containing a lowered level of polygalacturonase isoform 1 is disclosed. This method begins with the step of isolating a DNA sequence encoding at least a portion of the polygalacturonase beta-subunit. This portion is sufficient to hybridize effectively to the mRNA of the polygalacturonase beta-subunit in vivo. A genetic construction is created from the cDNA clone in which the cDNA clone is positioned so that the antisense version of the polygalacturonase beta-subunit message may be produced from the clone. A tomato cell is transformed with the construction, whereby the tomato cell produces in the fruit a lowered level of PG beta-subunit and, hence, lowered levels of polygalacturonase isoform 1.</p> |
申请公布号 |
EP0577252(A1) |
申请公布日期 |
1994.01.05 |
申请号 |
EP19930303533 |
申请日期 |
1993.05.07 |
申请人 |
ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA |
发明人 |
DELLAPENNA, DEAN |
分类号 |
A01H1/00;A01H5/00;A01H5/08;C12N1/20;C12N1/21;C12N9/24;C12N15/09;C12N15/82;C12R1/01;(IPC1-7):C12N15/82;C12N9/18;C12N15/11 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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