HIGHLY SENSITIVE DETERMINATION OF AMONIA, -g(a)-AMINO ACID OR -g(a)-KETO ACID AND COMPOSITION THEREFOR.

摘要

Determination of ammonia, alpha -amino acid, corresponding alpha -keto acid or ionic species thereof by reacting a specimen solution with a reagent containing 1) amino acid dehydrogenase, 2) A1, 3) B1, and 4) if necessary, other ingredient(s) required for forming the cycle shown in the drawing, and determining the amount of A2 formed or the amount of B1 consumed; and a composition containing the ingredients 1), 2), 3) and 4) for use in the above determination method. The method and the composition make it possible to determine a small amount of a specimen solution conveniently with high sensitivity and are useful in the field of clinical inspection and food testing.