| 发明名称 |
Human t-PA production using vectors coding for DHFR protein |
| 摘要 |
A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
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| 申请公布号 |
US5268291(A) |
申请公布日期 |
1993.12.07 |
| 申请号 |
US19910663103 |
申请日期 |
1991.02.28 |
| 申请人 |
GENENTECH, INC. |
发明人 |
LEVINSON, ARTHUR D.;PENNICA, DIANE;KOHR, WILLIAM J.;VEHAR, GORDON A.;GOEDDEL, DAVID V.;SIMONSEN, CHRISTIAN C. |
| 分类号 |
C12N9/06;C12N9/72;C12N15/58;C12N15/85;(IPC1-7):C12N5/10;C12N5/16;C12P21/02 |
主分类号 |
C12N9/06 |
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