Human IL-1 cDNA sequences encoding biologically-active human IL-1 proteins.

摘要

Nucleic acids comprise a nucleotide sequence encoding human interleukin-1 (IL-1), and fragments thereof, and the polypeptides and peptides obtained. Truncated human IL-1 cDNA sequences encode biologically-active novel human IL-1 proteins. cDNA synthesised by reverse transcription of poly(A)RNA isolated from adherent human monocytes stimulated with bacterial endotoxin, is cloned. Truncated human IL-1 cDNA sequences can be obtained by genetic engineering procedures using cloned human IL-1 cDNA as a starting material. Human IL-1 and human IL-1 proteins obtained via cloned truncated human IL-1 cDNA sequences can induce the production of IL-2 by activated T-cells; they also act on B-cells and NK-cells.