Electroosmotic flow control apparatus for capillary electrophoresis

摘要

An electrophoretic apparatus that is useful for separation of analytes, such as proteins, comprises a capillary tube that has an interfacial layer bonded to reduce interactions between the bore and analytes during electroosmotic flow through the capillary, and an electrically modified media carried by or contacting a capillary wall outward from the bore. The modified media can be an electrically resistive coating or an electrically conductive coating. Together with appropriate power supply configurations, the electrically modified media permits the application of a voltage gradient that is transverse to the electrophoretic gradient, and provides electroosmotic flow control in cooperation with the interfacial layer.