摘要 |
PURPOSE:To excellently separate basic compounds of amines which can be hardly separated from each other by using a mobile phase containing anions containing a plurality of fluorine atoms. CONSTITUTION:The anion which contains a plurality of fluorine atoms is that containing two or more fluorine atoms in one molecule of anion, such as the phosphor hexafluoride ion, arsenic hexafluoride ion, etc. Any mobile phase that can dissolve an ion-pair reagent at a required concentration and does not give any adverse influence to a stationary phase, sample, and chromatography equipment or mobile phase composed mainly of a nonpolar solvent, such as hexane, etc., can be used as the mobile phase to which anions are added. Such mobile phase is suitable for the separation of a mixture containing molecules which can produce anions, because the phase largely changes the intensity of retention in the chromatography of anions, such as amines, or molecules which can produce anions. Moreover, since the mobile phase has a similar structure, the phase can be used for the separation of a hardly separable mixture, such as an optical isomer, etc., containing a polysaccharide derivative as a stationary phase. |