| 摘要 |
A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer. To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end o f the target sequence, and a reserve transcriptase and an RNA polymerase are provided to a mixture including the target sequenc e; the amplification proceeds accordingly. The in- vention is useful for generating copies of a nucleic acid target sequence fo r purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
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